배양된 인간 골막기원세포의 조골활성에 대한 덱사메타손 농도의 효과
Effect of dexamethasone concentrations on osteogenic activity of cultured human periosteal-derived cells
김종렬, 성일용, 박봉욱, 이창일, 하영술, 김덕룡, 조영철, 변준호,
소속 상세정보
김종렬 ( Kim Jong-Ryoul ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
성일용 ( Sung Iel-Yong ) - 울산대학교 의과대학 구강악안면외과학교실
박봉욱 ( Park Bong-Wook ) - 경상대학교 의학전문대학원 구강악안면외과학교실
이창일 ( Lee Chang-Il ) - 경상대학교 의학전문대학원 구강악안면외과학교실
하영술 ( Hah Young-Sool ) - 경상대학교병원 임상의학연구소
김덕룡 ( Kim Deok-Ryong ) - 경상대학교 의학전문대학원 생화학교실
조영철 ( Cho Yeong-Cheol ) - 경상대학교 의학전문대학원 생화학교실
변준호 ( Byun June-Ho ) - 경상대학교 의학전문대학원 구강악안면외과학교실
KMID : 0360120090310040287
Abstract
Long-term treatment with glucocorticoid leads to the development of osteoporosis and osteonecrosis. In contrast to the marked inhibitory effect of pharmacological doses of glucocorticoids on bone formation, the relationship between physiological concentrations of glucocorticoids and osteoprogenitor cell proliferation and phenotypes has not been elucidated yet. In addition, the effects of dexamethasone treatment on the proliferation and osteoblastic differentiation of osteoprogenitor cells are also controversial. The purpose of this study was to examine the effects of dexamethasone on the proliferation and osteoblastic differentiation of periosteal-derived cells. Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the cells were further cultured for 21 days in the osteogenic induction medium with different dexamethasone concentrations of 0, 10, and 100 nM. The proliferation and osteoblastic phenotypes of periosteal-derived cells were promoted in dexamethasone-treated cells than in untreated cells. Among the dexamethasone-treated cells, cell proliferation was slightly greater in 10 nM dexamethasone-treated cells than in 100 nM dexamethasone-treated cells. Histochemical staining and the bioactivity of alkaline phosphatase (ALP) were higher in 100 nM dexamethasone-treated cells than in 10 nM dexamethasone-treated cells. Similarly, von Kossa-positive mineralization nodules and calcium content were also more evident in 100 nM dexamethasone-treated cells than in 10 nM dexamethasone-treated cells. These results suggest that dexamethasone enhances the in vitro osteoblastic differentiation of periosteal-derived cells. The present study also demonstrates that higher dexamethasone concentrations reduce the in vitro proliferation of periosteal-derived cells.
키워드
Periosteal-derived cell; Dexamethasone; Osteogenic activity
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